Figure 2.

DNA sequence of lgtG locus from GC strain 15253. RBS 1 and RBS 2 indicate two possible ribosome-binding sites (marked by double underlines) corresponding to two alternative translational starts (thick underlines), START 1 and START 2, respectively. poly(C) (black box) indicates the phase variable 11 consecutive Cs. The FA1090 sequence has 11 Cs also. The number of Cs in F62 and 1291 is 12 and the ORF terminates at STOP 2 instead of STOP 1, the putative translational terminator (white box) in 15253/FA1090. In MS11, 10 Cs are present and translation terminates at STOP 3. The locations of the relevant PCR primers are underlined by arrows. The ermC′ cassette was introduced into the indicated BstBI restriction enzyme site (italic font) for insertional inactivation of lgtG ORF. The NdeI sites were used to create pK18-LgtG18ΔNdeI. −10? and −30?, possible −10 consensus and −30 consensus of σ⁷⁰-binding site (gray boxes).