Involvement of lgtG and its
poly(C) tract on LOS biosynthesis of GC. (A) SDS-PAGE
analysis of LOS isolated from F62Δ8–1 and its derivatives. The lanes
represent LOS isolated from: 1, F62Δ8–1; 2, F62Δ8–1(3G9+); 3,
F62Δ8–1(3G9+)lgtG; and 4,
F62Δ8–1(3G9+)lgtG transformed with pK18-LgtG18 (Table
1). (B) DNA sequencing gel containing the C lanes of the
three derivatives of pK18-LgtG18. (C) Photoreproduction of
the region of a GC-agar plate containing spots of various
F62Δ8–1-derived cells. The small dots inside the spots represent
actual colonies after 24 hr of growth. Rows 1 through 3 represent cells
that were transformed with constructs that contained 10 Cs, 12 Cs, and
11 Cs, respectively, in poly(C). Row 4 represents cells that were
transformed with N. gonorrhoeae strain 4318 (Table 1)
chromosomal DNA. (D) Colony immunoblot of the cells shown in
C using mAb 3G9.