Table 1.

A list of strains and plasmids used

Strains/plasmids	Relevant genotype/phenotype/description	Source
N. gonorrhoeae strains
F62	Full LOS α chain but no β chain. Defective lgtG with 12 C poly(C)	12, 36
F62lgtG	F62 mutant* with ermC′(6) inserted in lgtG	This work
F62Δ5	lgtA-D deleted F62. Truncated lactosyl α chain	6
F62Δ8-1	239-bp ApoI fragment deleted in lgtA. Truncated lactosyl α chain	This work
F62Δ8-1(3G9+)	Spontaneous mutant of F62Δ8-1 that reacts with mAb 3G9	This work
F62Δ8-1(3G9+)lgtG	F62Δ8-1(3G9+) with lgtG deletion. Also see Fig. 3	This work
FA1090	Full LOS. Complete α and β chains. Functional lgtG with 11 C poly(C)	M. Cohen† and (36)
FA1090lgtG	NdeI fragment (538–632 in Fig. 2) deleted in FA1090 lgtG. No β chain	This work
15253	Full β but truncated lactosyl α chain. lgtB-D deleted and defective lgtA	5, 19, 37
15253lgtG	515253 without β chain due to ermC′ disruption in lgtG	This work
15253lgtE	lgtE mutant of 15253. Both α and β chain contain only one Glc each	19
1291	No β but full α chain. Similar to F62 LOS. 12 C poly(C) in lgtG	36
MS11	No β but full α chain like F62 and 1291. 10 C poly(C) in lgtG	J. Swanson‡ and ref. 37
4318	Reacts with mAb 3G9. β chain present	P. F. Sparling†
Plasmids
pLgtG7	2.3-kb Tsp509I insert with 15253 lgtG in EcoRI site of pBluescriptII SK(+)	This work
pLgtG7-erm	Klenow enzyme-treated NotI fragment carrying ermC′ of pHSS6-erm (6)  ligated with BstBI digested and T4DNA polymerase-treated pLgtG7	This work
pK18	GC uptake sequence cloned into BglII site of E. coli vector pK18 (38)	38
pK18-LgtG18	pK18 with DNA fragment isolated from strain 4318	This work
pK18-LgtG18ΔNdeI	94-bp NdeI deletion (538–632 of Fig. 2) in lgtG of pK18-LgtG18	This work

^*All mutants are isogeneic. 

^†University of North Carolina, Chapel Hill, NC. 

^‡Rocky Mountain Laboratory, MT.