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In vitro selection scheme using mRNA display. The starting dsDNA pool (top, center) which encodes the peptide library is transcribed in vitro. Purified mRNA is enzymatically ligated to a puromycin-DNA oligo prior to RNA-peptide fusion formation via in vitro translation. Purified RNA-peptide fusions are reverse transcribed and affinity selected onto the immobilized antibody target. Eluted cDNA is used as the template for PCR for the next cycle of selection.
