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. 2010 Jan;20(1):133–141. doi: 10.1101/gr.100396.109

Figure 3.

Figure 3.

Cis-antisense transcription in S. solfataricus. Green TSS positions represent transcripts encoded from the strand opposite to the protein-coding gene. Read coverage is in log scale. (A) Antisense transcription in the SSO1457 locus, encoding NAD-specific glutamate dehydrognase. (B) Antisense transcription in the SSO2178 locus, encoding aspartate semialdehyde dehydrognase. (C) Antisense transcription in the SSO2127 locus, encoding a sugar permease (sense transcription not observed). (D) Antisense transcription in the SSO0871 locus, encoding ornithine carbamoyltransferase. (E) Antisense transcription in the SSO1032 locus, encoding a sulfate ABC transporter (sense transcription not observed). (F) Antisense transcripts have promoters embedded within the protein-coding genes they overlap. Sequence motifs upstream of protein-coding genes (upper panel) compared to motifs upstream of noncoding antisense transcripts (lower panel). Positions relative to TSS are marked below the sequences. The height of each letter corresponds to its frequency at that position. The core promoter motif in Archaea is indicated. The plot was prepared using the WebLogo software tool (Crooks et al. 2004).