Skip to main content
letter
. 2010 Jan;20(1):19–27. doi: 10.1101/gr.100073.109

Figure 4.

Figure 4.

Characterization of RnERV-K8e-specific transcripts. (A) Quantitative TaqMan PCR analysis of RnERV-K8e transcription in different tissues of BN rats, with primers specific for the gag or env genes. Heart, brain (frontal lobe), and testis samples were collected from three males; ovaries were obtained from two females. The three midgestation (14.5 dpc) embryos were littermates. Each sample was run in six replicates, including the GAPDH control. Columns represent mean values, error bars show the standard error of the mean (SEM) (for statistical comparison see Supplemental material). Expression in testis is set to 1. (B) Polymorphic status of RnERV-K8 by Northern blotting of total RNA from testes of SHR+/+, BN+/+, WHD+/+, and WKY strains. Probe corresponds to gag (left panel) and env (right panel) fragment of the Cntrob-ERV, respectively. A <9-kb transcript (corresponding to the full-length of RnERV-K8e) is detectable in all the samples. The specific bands have various intensities in the different strains. Notably, the predicted full-length transcript detected by the env probe is highly abundant in BN testis.