Figure 5.

Analyses of clonal populations of cells whose genomic segments were deleted by ZFNs. (A) Schematic of CCR2 and CCR5 loci in wild-type cells and in clonal cells whose genomic segments were deleted. The probe (black bar) and the restriction enzyme, XbaI, used for Southern blot analysis are indicated. F2, R2, F5, and R5 are PCR primers (arrows) used for the detection of genome deletion events or the intact CCR2 and CCR5 loci. (B) PCR products validating genomic deletions and the intact CCR2 and CCR5 region. PCR primers are shown in parentheses. Clones 1 to 4 are clonal cells isolated by limiting dilution. (WT) Wild-type cells. (C) DNA sequences of deletion junctions in clonal cells. Symbols are as in Figure 1. (D) Southern blot analysis of clonal cells.