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. 2009 Dec;59(6):589–597.

Figure 5.

Figure 5.

RAPD-PCR banding pattern of a subset of isolates from NHP infected with HMV K. pneumoniae. Isolates were identified as being HMV K. pneumoniae based on Vitek analysis and a positive string test. “M” indicates a 1 kb plus marker, and NTC indicates a negative control. The isolate labeled AGM was an HMV K. pneumoniae culture from a fatal abdominal abscess in an African green monkey in 2006. All other isolates were from oropharyngeal or rectal swabs from asymptomatic macaques. Animal identifications correlate with those in Tables 1 and 2. Isolates labeled Cy4a and Cy4b represent 2 rectal samples taken from NHP Cy4 about 3 wk apart (Cy 4a, 2008 Apr 28; Cy4b, 2008 May 20). Animals from Vendor 1 comprised a single shipment, whereas NHP that came from Vendor 2 arrived in 3 separate shipments, as indicated. The remaining 2 macaques were transferred from 2 different Department of Defense research centers, indicated as Research Institute 1 (Res Ins 1) and Research Institute 2 (Res Ins 2). PCR results were correlated with the RAPD-PCR banding patterns. Isolates were determined to be rmpA+/magA− (*), rmpA−/magA+ (†), or rmpA−/magA− (‡). Clusters A through D indicate distinct RAPD types, which were determined based on the absence or presence of 2 or more bands.