Table 1.
Number of transcripts significant at 1% FDR
| Location | Gender | Interaction | ||||||
|---|---|---|---|---|---|---|---|---|
| ANOVA | ANCOVA | ANOVA | ANCOVA | ANOVA | ANCOVA | |||
| 3-way | 8459 | 7057 | Male : Female | 151 | 233 | Location*Gender | 133 | 203 |
| Aga : Bou | 6744 | 4974 | In Agadir | 24 | 24 | Fem (Aga : Bou) | 4830 | 3791 |
| Aga : Igh | 635 | 651 | In Boutroch | 13 | 14 | Fem (Aga : Igh) | 1451 | 1467 |
| Bou : Igh | 7339 | 6286 | In Ighrem | 589 | 890 | Mal (Aga : Bou)† | 407 | 806 |
| Aga : Rural | 1521 | 607 | Mal (Aga : Igh) | 8 | 8 | |||
ANOVA includes terms for Location, Gender, and Location*Gender interaction. The False Discovery Rate was evaluated using the conservative Benjamin and Hochberg method. The left hand columns show the number of genes significant at the 1% FDR threshold for Location effects (either in the 3-way comparison of Agadir (Aga), Boutroch (Bou) and Ighrem (Igh); between pairs of locations, or between Agadir (Aga) and the two rural sites combined). The central columns contrast gender (male versus female) effects, either in the total sample or each location individually. The right hand columns show interaction effects, either in the total sample or showing the indicated contrast between Agadir and either village, for females or males separately. ANCOVA is the same model with an additional continuous covariate for ethnicity, genotypic PC2.
Significance of this contrast was reduced by the small sample of Boutroch males (12, cf 26 females).