Fig. 8.

Effect of TUDCA on LIRD mouse retina caspase-3 activation. Mice were subcutaneously injected with either vehicle or TUDCA (500 mg/kg), exposed to 200 (dim) or 10,000 lx (bright) of white light for 7 h, then returned to maintenance lighting conditions. Mice were killed 24 h later, and paraffin retina sections were prepared and probed for activated caspase 3 immunoreactivity by fluorescent confocal microscopy. Representative composite confocal micrographs for vehicle dim-light-exposed vehicle-injected (left), bright-light-exposed vehicle-injected, and bright-light exposed TUDCA-injected (right) treatments are shown. There was significantly more immunoreactivity (yellow signal) in sections of bright-light-exposed vehicle-treated mice than in bright-light-exposed TUDCA-treated mice or dim-light-exposed mice. Image reprinted with permission from Ref. [81]