Figure 1.

Ad-TrkB.T1-GFP inhibits BDNF-induced phosphorylation of TrkB. (a) Viral constructs: In Ad-TrkB.T1-GFP, a truncated TrkB receptor expression cassette was inserted into the E1 region of an adenovirus vector containing a GFP expression cassette in the E3 region. In the control Ad-ΔE1-GFP, the GFP gene was inserted into the E3 region of an adenovirus vector deleted in the E1 region. (b) Antiphosphotyrosine blots of anti-TrkB immunoprecipitated fractions from Chinese hamster ovary cells infected with Ad-TrkB and Ad-ΔE1-GFP. The full-length TrkB receptor is detected near 130,000. Treatment with Ad-TrkB.T1-GFP substantially reduced the amount of phosphorylated TrkB receptor detected.