TABLE I.
Primer Name, Primer Sequence, Repeat Motif (R), Annealing Temperature (Ta), Predicted Product Size (Ps), Actual Allele Size (As), Number of Alleles (Na) and Heterozygosity (Ho)in Callithrix jacchus
| Primers | Sequence (5′–3″) | R | Ta (°C) | Ps | As | Na | Ho |
|---|---|---|---|---|---|---|---|
| CAJA1F | 6FAM-GAAGACGGGGGCGTAAATA | (CA)23 | 55 | 382 | 383, 385, 387, 389, 391 | 5 | 0.74 |
| CAJA1R | TGTGGTGGCTCATACCTGAA | ||||||
| CAJA5F | PET-GGCCCACAGCATTTAATTTG | (GT)18 | 48 | 240 | 246, 248, 250 | 3 | 0.63 |
| CAJA5R | CAACTTTACTGCCACCAGCA | ||||||
| CAJA6F | NED-GAGCACCAAGATTGGCATTT | (GT)15 | 48 | 220 | 220, 222, 224, 226, 228, 230 | 6 | 0.72 |
| CAJA6R | CCAATACACATCGGCTTTGA | ||||||
| CAJA9F | 6FAM-GCTCCCAGACACAAGCTCAT | (GT)13 | 55 | 182 | 188, 190, 200 | 3 | 0.51 |
| CAJA9R | TGCTTTTCTTCTGCCCAATC | ||||||
| CAJA10F | VIC-ACCCTACATTGCCAAATTGC | (CA)22 | 55 | 190 | 178, 186, 188, 192, 194, 196, 198, 200, 203 | 9 | 0.82 |
| CAJA10R | GCCTCTTCTGAGGGAAGTGA | ||||||
| CAJA11F | 6FAM-CGAAAGTGTGCTCAACAGGA | (CA)13 | 48 | 243 | 243, 245, 251, 253, 256, 258 | 6 | 0.61 |
| CAJA11R | AAGGTGGGATTCTGAAAGCA | ||||||
| CAJA12F | VIC-GCCAACGAGAGGCTCTAATG | (GT)17 | 48 | 228 | 229, 231, 233, 237, 240, 243, 245 | 7 | 0.83 |
| CAJA12R | TTTTGCTTGTGGCAATGGTA | ||||||
| CAJA13F | NED-TGAGCCAACGTACCTGGTTA | (GT)17 | 48 | 358 | 365, 367, 369, 371, 375 | 5 | 0.64 |
| CAJA13R | CTTTTCCAATGCGAGAGGAG | ||||||
| CAJA14F | 6FAM-AGCACATGAACACCCAGGTT | (CA)16 | 57 | 213 | 201, 204, 215, 217, 219 | 5 | 0.57 |
| CAJA14R | AGTGAAAACAGGCTGGGAGA | ||||||
| CAJA15F | PET-ACCACATGATCCAGCAATCC | (CA)23 | 57 | 153 | 133, 135, 137, 145, 147, 150, 153, 155, 157 | 9 | 0.80 |
| CAJA15R | GATTCCGTGTCTTAGCCATCA | ||||||
| CAJA16F | NED-AGGGCCTTCCACAGAGTGTA | (GT)11 | 48 | 386 | 365, 367, 371, 388, 391, 396 | 6 | 0.48 |
| CAJA16R | CCTCTGCACTCTTCCTTTGG | ||||||
| CAJA17F | PET-GGGCACTCCAAGGTCAGTAA | (CT)21 | 57 | 377 | 315, 325, 337, 348, 371, 373, 375, 380, 382 | 9 | 0.81 |
| CAJA17R | TTGCCCCCTGCTTATTGTAG | ||||||
| CAJA18F | VIC-ACTTGCAGGCCAGTGTTCTT | (AC)17 | 48 | 303 | 288, 290, 296, 302, 306, 308, 317 | 7 | 0.68 |
| CAJA18R | TGGACAGCTGAGGTTTCCTT | ||||||
| CAJA19F | 6FAM-AGTTCTCCGAGCTCCTCCTC | (CA)13 | 48 | 356 | 329, 338, 346, 360, 367, 373 | 6 | 0.70 |
| CAJA19R | TGGGTGATTTTCATCCCTGT |
6FAM, PET, NED and VIC are florescent dyes attached to the primers.