Fig. 2.

Ngn3-dependent expression of Rfx6 in the mouse islet lineage. (A-F) Double immunofluorescence showing partial overlapping expression of Ngn3 (red) and Rfx6 (green) at E10.5 (A-C) and E15.5 (D-F). Yellow, green and red arrows point to double-labelled, single Rfx6-positive and single Ngn3-positive cells, respectively. Nuclei are stained with DAPI. (G,H) Loss of Rfx6 expression (in situ hybridization in blue) in an Ngn3-deficient pancreas (H) compared with a wild-type pancreas (G), demonstrating that Rfx6 is restricted to the islet lineage at E15.5.