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. 2010 Jan 15;137(2):283–292. doi: 10.1242/dev.040287

Fig. 3.

Fig. 3.

RA is synthesized by both the ureteric bud and mesenchyme in the embryonic kidney. (A-C) LacZ expression in F9-RARE;lacZ reporter cells cultured for 16 hours in medium without added retinoic acid (RA) (A), with 10–8 M at 9-cis RA (B) and with 10–7 M retinol (C), an inactive RA precursor. (D-G) LacZ expression in explants cultured for 16 hours on lawns of F9-RARE;lacZ reporter cells: E11 mesenchyme without a source of vitamin A (retinol) (D), E11 mesenchyme with 10–7 M retinol (E), E11 ureteric buds plated without retinol (F) and E11 ureteric buds with 10–7 M retinol (G). The schematic on the right shows separated metanephric mesenchyme (purple, assayed for lacZ activity in 3D,E) and ureteric bud explants (yellow, assayed for lacZ activity in 3F,G). Magnification: 20× in A-G.