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. 2010 Jan 15;137(2):283–292. doi: 10.1242/dev.040287

Fig. 4.

Fig. 4.

RA is sufficient for maintaining Ret expression and branching of isolated ureteric buds in the absence of mesenchyme. (A) A wholemount E11 ureteric bud prior to culture. (B) E11 ureteric buds cultured in serum-free medium including Gdnf, with added retinoic acid (RA). (C) In situ hybridization showing Ret expression in ureteric buds cultured for 48 hours in medium containing Gdnf and RA. (D) A wholemount ureteric bud cultured for 48 hours in medium with Gdnf and without added RA. (E) Undetectable Ret expression in an E11 ureteric bud cultured for 48 hours in medium with Gdnf and without RA. Magnification: 20× in A-E.