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. 2010 Jan 15;137(2):283–292. doi: 10.1242/dev.040287

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Fig. 6. — RA signaling is important for primary ureteric bud formation as well as for branching morphogenesis within the kidney. (A,B) Ureteric bud formation in a wholemount E10.5 control (Hoxb7-Cre^–/–;RaraDN^flox/flox;Hoxb7-Gfp) embryo (A) and an E10.5 Hoxb7-Cre^–/+;RaraDN^flox/flox;Hoxb7-Gfp embryo expressing two alleles of the RaraDN transgene (B). (C,D) Gfp expression in an E11.5 control embryo (C) and an E11.5 Hoxb7-Cre^–/–;RaraDN^flox/flox;Hoxb7-Gfp embryo (D). (E,F) Wholemount in situ hybridization showing Ret expression in the emerging ureteric bud of a control (Hoxb7-Cre^–/–;RaraDN^flox/flox) E10.5 embryo (E) and an E10.5 Hoxb7-Cre^–/+;RaraDN^flox/flox;Hoxb7-Gfp embryo (F). The black arrows point to the caudal Wolffian ducts. Magnification: 20× in A-F.