Figure 4.

Distinct morphologies of ES cells cultured in the acellular kidney scaffold. After 10 d of whole organ culture (PAS stain, sodium deoxycholate protocol): cells filling the glomerular capillary tufts (A) (×1250) and flat-appearing cells lining vascular structures (B) (×1500). After thick section culture (H&E stain, SDS protocol): at day 6, apoptosis of cells in vessel lumens (C) (×400, a higher magnification of a field in Figure 3B); at day 8, cell aggregates within artery (D) (×400); at day 10, cells lining an artery (E) (×400); and at day 10, solid round cellular aggregates with central apoptosis (F) (×400).