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. 2009 Nov 6;10(12):1355–1362. doi: 10.1038/embor.2009.233

Figure 1.

Figure 1

Analysis of Asef and Asef2 expression levels in colon cancer tissues. (A) Quantitative analysis of Asef, Asef2, MMP9 and axin 2 expression in human colon cancerous and corresponding non-cancerous tissues by real-time RT–PCR. Asef, Asef2, MMP9 and axin 2 mRNA expression was quantified as the percentage relative to GAPDH mRNA (n=19 pairs). Filled circles represent cancerous tissues in which the indicated mRNA is expressed at a higher level than in the corresponding non-cancerous tissues. For Asef, the open circles in the bottom row (Neg) represent tissues in which Asef expression was not detectable. Axin 2 was used as a marker of β-catenin–TCF-mediated activation. *P<0.05; **P<0.01; ***P<0.001. (B) Immunohistochemical analysis of Asef/Asef2 on sections from human colon cancer tissues and adjacent normal mucosa. The antibody used here recognizes both Asef and Asef2. Scale bar, 50 μm. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mRNA, messenger RNA; RT–PCR, reverse transcriptase–PCR; TCF, T-cell factor.