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. 2009 Nov 6;10(12):1348–1354. doi: 10.1038/embor.2009.236

Figure 4.

Figure 4

TM9SF4 silencing and phagocytosis. (A) FACS analysis of TM9SF4 expression in untransfected, SC-siRNA-transfected or TM9SF4 siRNA-transfected MM2 cells. (B) FACS analysis of phagocytic activity in untransfected, SC-siRNA-transfected or TM9SF4 siRNA-transfected MM2 cells incubated with FITC-stained yeast cells. (C) FACS analysis of cannibal activity in untransfected, SC-siRNA-transfected or TM9SF4 siRNA-transfected MM2 cells incubated with DHR123-stained lymphocytes. Only melanoma cell fluorescence emission was evaluated. Numbers reported are the percentage of cells containing FITC-stained yeast cells or DHR123-stained lymphocytes. DHR123, dihydrorhodamine 123; FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; MM, metastatic melanoma; SC, scrambled; siRNA, short interfering RNA; TM9SF4, transmembrane 9 superfamily protein member 4.