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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: Nature. 2009 Dec 10;462(7274):808–812. doi: 10.1038/nature08612

Figure 3. Mutant enzyme with a smaller pocket retains serine binding affinity.

Figure 3

a-c, Kinetic parameters for amino acid activation of G237A and WT AlaRS441. kcat/KM ratios of noncognate serine and glycine were calculated against cognate alanine for each protein, separately. Error bars are s.d., n ≥ 3.

d-f, G237A AlaRS441-LZ structures in complex with three different ligands are compared with the corresponding WT AlaRS441-LZ complex structures (white). For clarity, only the active site cavities of WT AlaRS441-LZ structures are shown here.