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. Author manuscript; available in PMC: 2009 Dec 29.
Published in final edited form as: Ann N Y Acad Sci. 2005 May;1041:296–299. doi: 10.1196/annals.1282.044

FIGURE 1.

FIGURE 1

Relaxin fails to increase cAMP in MCF-7 cells. Duplicate samples (5 × 104 cells per treatment) were pretreated in the presence of phosphodiesterase inhibitor (50 μM isobutyl-1-methylxanthine, IBMX) for 15 minutes and then treated either with vehicle or with 0.1 μg/mL relaxin in the presence or absence of 3, 30, or 100 nM forskolin (Fsk) for 20 minutes. Treatments were stopped by removal of media and addition of 0.1 N HCl. Intracellular cAMP was detected by enzyme immunoassay. Data are expressed as the mean ± SE from a single experiment and are representative of three different experiments.