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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Radiother Oncol. 2009 Oct 23;93(3):639–644. doi: 10.1016/j.radonc.2009.09.006

FIG. 2. Radiosensitization by lapatinib is mediated primarily through inhibition of MEK.

FIG. 2

(A) SUM102 cells were grown to 80% confluence in complete medium and treated with CI1040 (1 μM), SP600125 (20 μM) or vehicle (DMSO) 2 h prior to irradiation at 5 Gy. Protein lysates were collected at 0-90 min after irradiation and western blot analyses performed with the indicated antibodies. (B) SUM102 cells were treated with SP600125 (20 μM), CI-1040 (1 μM), lapatinib (2.5 μM ) or vehicle (DMSO) 2 h prior to irradiation at 5 Gy and the media replaced without drug and colonies counted after 2 weeks. Surviving fractions of each treatment were normalized to cells treated with radiation alone (5 Gy). * indicates p<0.001 by unpaired t-test.