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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Dev Dyn. 2009 Jan;238(1):138–149. doi: 10.1002/dvdy.21833

Figure 7. The differentiation-resistant cells demonstrate characteristics of a progenitor cell population.

Figure 7

Differentiation-resistant cells were isolated from fetal multinucleated myotubes as detailed in the material and methods section and then re-expanded to form myotubes or plated at low density for clonal analysis. (A) Differentiation-resistant cells that were expanded and induced to differentiate a second time were immunostained for Pax7 and desmin. The staining showed that differentiation-resistant cells could form myotubes but had a coordinated loss of Pax7+ cells (arrow). (B,C,D) Clonal analysis of the differentiation-resistant cells revealed three types of colonies that formed with respect to Pax7 and desmin expression. The first image of each row shows a 10X view of the colony using DAPI exposure. (B) Example of a colony where all cells within the colony were Pax7+/desmin−. (C) Example of a rare colony showing cells that were Pax7+/desmin− (arrowhead) as well as cells that were Pax7+/desmin+ (arrow). (D) Example of a colony where all cells were Pax7−/desmin−. Scale Bar = 20μM

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