Table III.
Values of Ki,app for Pi in the presence of 3-PGA, F-6-P, and G-6-P
The activator concentrations were chosen on the basis of the respective A50 values (Table I).
| Enzyme | Mutant Subunit | 3-PGA |
F-6-P |
G-6-P |
|||
|---|---|---|---|---|---|---|---|
| [3-PGA] | Ki,app | [F-6-P] | Ki,app | [G-6-P] | Ki,app | ||
| mm | mm | mm | |||||
| Wild type | – | 2.5 | 1.8 ± 0.3 | 5.0 | 1 ± 0.4 | 25 | 1.1 ± 0.4 |
| R104A | Large | 40 | 6.59 ± 0.06 | 10 | 7.7 ± 0.2 | 40 | 5.49 ± 0.07 |
| R77K | Small | 15 | 9 ± 3 | 100 | 5.4 ± 0.1 | 100 | 9.7 ± 0.1 |
| R116A | Large | 8.0 | 1.5 ± 0.1 | 14 | 1.1 ± 0.2 | 60 | 3.96 ± 0.09 |
| R107A | Small | 12 | 2.4 ± 0.1 | 3.0 | 4.3 ± 0.7 | 20 | 11.73 ± 0.03 |
| R146A | Large | 15 | 5.0 ± 0.1 | 30 | 14.1 ± 0.2 | 60 | 13.9 ± 0.1 |
| R340A | Small | 35 | 21.93 ± 0.06 | 70 | 60 ± 15 | 100 | 28.33 ± 0.09 |
| R381A |
Large |
20 |
1.1 ± 0.4 |
30 |
2.4 ± 0.3 |
100 |
5.18 ± 0.08 |