Identity of MVP1. A, A schematic drawing of MVP1 shows the mvp1-1 point mutation in the first exon of At1g54030 and a second insertional allele, mvp1-2, also in the first exon. B, RT-PCR analysis of MVP1 and ACTIN7 control transcript levels in parental GFP:δ-TIP (δ-TIP), mvp1-1, wild-type Col-0, and mvp1-2. C, Expression of the wild-type MVP1 gene under control of its native promoter in mvp1-1 mutant plants restores normal vacuolar architecture, as shown here in hypocotyls. Bars = 50 μm.