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. 2010 Jan;152(1):133–150. doi: 10.1104/pp.109.146381

Figure 3.

Figure 3.

Subcellular localization of FsPP2C1, PYL8/RCAR3 (At5g53160), and the protein interactions between FsPP2C and PYL/RCAR proteins determined in leaf epidermis of tobacco plants. A, Transiently transformed epidermal onion cells. Confocal microscopy slides of cell sections show the exact localization of the GFP-FsPP2C1 and GFP-At5g53160 proteins in epidermal onion cells. B, Agroinfiltration analysis in tobacco leaves. Constructs delivered in both cases correspond to N-terminal GFP fusions of full-length FsPP2C1 (35S:GFP-FsPP2C1) and At5g53160 (35S:GFP-At5g53160). Nuclear and cytosolic distribution of the GFP protein alone is shown as a control (35S:GFP). C, Constructs used in the transformation of tobacco. LB, Left border; RB, right border. D, BiFC detection of protein-protein interactions in subcellular locations. The interactions between YFPn-At5g53160 and YFPc-FsPP2C1 (top), YFPn-At4g01026 and YFPc-FsPP2C1 (middle), and YFPn-At5g05440 and YFPc-FsPP2C1 (bottom) are shown. E, Confocal microscopy images showing the exact localization of the FsPP2C1-At5g53160 interaction in the nucleus in the absence and presence of ABA.