Temporal and spatial expression of AtTZF1. A, RNA gel-blot analysis indicates that AtTZF1 mRNA is differentially expressed in different tissues. B, GUS staining showing differential expression of PAtTZF1:GUS in root tips and vasculature. Bar = 2 mm. C, Enlarged view of shoot apical region showing GUS stain in young leaf primordia. Bar = 200 μm. D, Enlarged view showing GUS stain in root tips and vasculature. Bar = 200 μm. E, PAtTZF1:GUS is expressed in anthers and female gametophytes (arrowheads) before pollination. Bar = 0.5 mm. F and G, Differential expression of PAtTZF1:GUS during embryo development. Shown are high levels of PAtTZF1:GUS expression in early stages of embryo development (F; bar = 0.5 mm) and very low to no expression after bending-cotyledon stages (G; bar = 2.5 mm).