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. 2010 Jan;152(1):151–165. doi: 10.1104/pp.109.145656

Figure 7.

Figure 7.

AtTZF1-associated cytoplasmic foci are present in etiolated seedlings and can be induced by wounding. A, AtTZF1-GFP is highly abundant in leaf primordia (LP), shoot apical meristem (SAM), and vasculature (V). B, In contrast to the fully expanded cotyledons, where AtTZF1-GFP is localized mainly in the vasculature, it is present in all cells in the true leaf primordia. In addition, AtTZF1-GFP cytoplasmic foci can be induced in the cells surrounding a wound (W) pinched by a pair of fine-tip forceps. Bar = 100 μm. C, Enlarged view is shown from the red rectangular area in B, a leaf primordium with numerous cytoplasmic foci. Some cytoplasmic foci are indicated by arrows. Bar = 20 μm. D, Enlarged view is shown from the red rectangular area in B, showing an induction of cytoplasmic foci in the cells surrounding a wounding site (bottom right). Some cytoplasmic foci are indicated by arrows. Bar = 10 μm. E, In etiolated cotyledons, AtTZF1-associated cytoplasmic foci are especially distinct in stomatal precursor cells (SPCs), in young guard cells (YGCs), and in stomatal-lineage ground cells (SLGCs) but are not apparent in mature stomata (MS). Bar = 30 μm.