Figure 3.

Daily transpiration rate, g_s, and A_N for grafted plants under normal and 100 mm NaCl irrigation. A and B, Whole-plant mean daily transpiration rate normalized to leaf area of all reciprocal grafted TOM-NtAQP1 and control plants measured simultaneously under normal irrigation (A) and after 3 d of 100 mm NaCl irrigation (B; T/T n = 3, C/C n = 3, C/T n = 8, T/C n = 6). se is given for each 10th sampling point; when not seen, se is smaller than the line. The green area represents morning hours, 8:00 to 11:00 am, and the light-red area represents noon hours, 11:00 am to 2:00 pm. At these hours and under the same salt conditions, parallel measurements of reciprocal grafted scions were conducted for g_s and leaf A_N, during the morning hours (C and E) and the noon hours (D and F). Bars represent means ± se of grafted plants (white for TOM-NtAQP1 and black for control; T\T n = 3 for morning and noon, C/C n = 4 for morning and n = 11 for noon, C/T n = 6 for morning and n = 5 for noon, T/C n = 5 for morning and n = 6 for noon). Different letters indicate significant differences (t test, P < 0.05). All measurements were performed in a semicontrolled greenhouse (see “Materials and Methods”); gas-exchange measurements were conducted with a portable apparatus (Li-6400) on young, fully expanded leaves.