Figure 3.

ISO-treatment and exercise induce enlargement of cardiomyocytes. A) 2-dimensional cell surface area of stained cardiomyocytes was measured using ImageJ software. At least 5 images were taken of the left-ventricle of three different hearts (for each group). Bars represent mean ± S.E.M of at least 400 cells. Asterisks indicate statistical significance after application of one-way ANOVA (p value < 0.0001). H&E stained sections of the left ventricle of a representative sedentary (B), ISO-treated (C), and exercised (D) mouse are also shown. Black scale bar = 60 μm.