Table 1.
Amino acid composition of the whole protein extract for all the conditions and strains tested
| Pichia pastoris | |||||||
| % mol/mol | Fab-expressing Strain | Control strain | Saccharomyces cerevisiae * | ||||
| 21% | 11% | 8% | 21% | 11% | 8% | ||
| Asx | 8.82 | 9.78 | 9.59 | 8.78 | 9.51 | 9.78 | 9.28 |
| Thr | 5.88 | 5.98 | 5.78 | 5.77 | 5.90 | 6.16 | 5.57 |
| Ser | 6.26 | 6.65 | 6.73 | 6.43 | 6.76 | 6.90 | 5.33 |
| Glx | 17.81 | 16.24 | 15.64 | 18.55 | 16.19 | 14.33 | 15.48 |
| Pro | 3.83 | 4.08 | 4.47 | 3.67 | 4.00 | 4.76 | 4.22 |
| Gly | 6.86 | 7.34 | 7.42 | 7.12 | 7.28 | 7.53 | 8.89 |
| Ala | 10.40 | 9.92 | 10.13 | 10.68 | 9.74 | 9.88 | 9.77 |
| Val | 5.88 | 6.05 | 6.09 | 5.58 | 6.78 | 6.16 | 7.33 |
| Cys | 0.15 | 0.15 | 0.14 | b.d. | 0.22 | 0.13 | 0.14 |
| Met | 0.79 | 0.67 | 0.78 | 0.77 | 0.80 | 0.70 | 1.14 |
| Ile | 4.64 | 4.68 | 4.76 | 4.12 | 4.45 | 4.70 | 5.89 |
| Leu | 6.96 | 7.26 | 7.46 | 6.99 | 7.23 | 7.94 | 8.01 |
| Tyr | 2.16 | 2.22 | 2.27 | 2.13 | 2.21 | 2.47 | 1.96 |
| Phe | 3.20 | 3.30 | 3.33 | 3.03 | 3.21 | 3.37 | 3.76 |
| Orn | 1.04 | 1.02 | 0.86 | 1.53 | 1.21 | 0.93 | 0.24 |
| Lys | 6.41 | 7.07 | 6.93 | 6.33 | 7.03 | 6.93 | 6.57 |
| His | 1.89 | 1.87 | 1.87 | 1.79 | 1.78 | 1.89 | 1.93 |
| Trp | 1.40 | 1.40 | 1.40 | 1.40 | 1.40 | 1.40 | 1.96 |
| Arg | 7.04 | 5.72 | 5.76 | 6.74 | 5.68 | 5.45 | 3.86 |
Amino acid composition measured for the different experimental conditions (% O2 in the inlet gas: 21, 11, 8) and for the different strains. Data given as percentage of each amino acid in the total pool of amino acids. * Data from Lange and Heijnen 2001 [11] for S. cerevisiae. Glx represents the pair Glu/Gln. Asx represents the pair Asp/Asn. b.d: below detection limit.