Table 2.
Average amino acid composition of the whole protein extract between the two strains for the different experimental conditions tested
| Pichia pastoris | Saccharomyces cerevisiae * | |||
|
% mol/mol |
21% | 11% | 8% | |
| Asx | 8.67 | 9.50 | 9.55 | 9.28 |
| Thr | 5.74 | 5.85 | 5.88 | 5.57 |
| Ser | 6.25 | 6.61 | 6.72 | 5.33 |
| Glx | 17.91 | 15.98 | 14.76 | 15.48 |
| Pro | 3.69 | 3.98 | 4.55 | 4.22 |
| Gly | 6.89 | 7.21 | 7.36 | 8.89 |
| Ala | 10.39 | 9.69 | 9.86 | 9.77 |
| Val | 5.65 | 6.32 | 6.03 | 7.33 |
| Cys | 0.15 | 0.18 | 0.13 | 0.14 |
| Met | 0.76 | 0.73 | 0.73 | 1.14 |
| Ile | 4.32 | 4.50 | 4.66 | 5.89 |
| Leu | 6.87 | 7.14 | 7.59 | 8.01 |
| Tyr | 2.11 | 2.18 | 2.33 | 1.96 |
| Phe | 3.07 | 3.21 | 3.30 | 3.76 |
| Orn | 1.27 | 1.10 | 0.88 | 0.24 |
| Lys | 6.28 | 6.94 | 6.83 | 6.57 |
| His | 1.81 | 1.80 | 1.86 | 1.93 |
| Trp | 1.38 | 1.38 | 1.38 | 1.96 |
| Arg | 6.79 | 5.62 | 5.52 | 3.86 |
Amino acid composition measured for the different experimental conditions (%O2 in the inlet gas: 21, 11, 8). Data given as percentage of each amino acid in the total pool of amino acids. * Data from Lange and Heijnen 2001 [11] for S. cerevisiae. Glx represents the pair Glu/Gln. Asx represents the pair Asp/Asn.