Table 5.
Fab-expressing strain | Control strain | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
mmol/gDW·h | 21% | 11% | 8% | 21% | 11% | 8% | ||||||
sd | sd | sd | sd | sd | sd | |||||||
Glucose | -0.99 | 0.05 | -1.33 | 0.07 | -1.74 | 0.09 | -0.99 | 0.05 | -1.32 | 0.07 | -1.92 | 0.10 |
OUR | -2.12 | 0.17 | -1.57 | 0.08 | -0.54 | 0.10 | -2.12 | 0.17 | -1.66 | 0.33 | -0.28 | 0.01 |
CER | 2.37 | 0.19 | 2.03 | 0.10 | 1.65 | 0.08 | 2.37 | 0.19 | 2.09 | 0.42 | 1.35 | 0.27 |
Biomass | 3.01 | 0.30 | 3.71 | 0.37 | 3.73 | 0.19 | 3.59 | 0.36 | 3.70 | 0.37 | 3.76 | 0.38 |
Ethanol | - | - | 0.33 | 0.02 | 1.00 | 0.05 | - | - | 0.31 | 0.02 | 1.16 | 0.06 |
Glycerol | - | - | 0.01 | 0.001 | 0.02 | 0.001 | - | - | 0.02 | 0.001 | 0.02 | 0.001 |
Citrate | 0.03 | 0.002 | 0.02 | 0.001 | 0.04 | 0.002 | 0.01 | 0.001 | 0.02 | 0.001 | 0.05 | 0.02 |
Arabinitol | - | - | 0.21 | 0.01 | 0.42 | 0.02 | - | - | 0.10 | 0.01 | 0.40 | 0.13 |
Pyruvate | - | - | 0.06 | 0.003 | 0.09 | 0.005 | - | - | 0.06 | 0.002 | 0.12 | 0.01 |
% C Balance Error excluding arabinitol | 6.4% | 15.7% | 23.9% | 1.3% | 14.5% | 29.3% | ||||||
% C Balance Error including arabinitol | 6.4% | 2.5% | 3.7% | 1.3% | 8.2% | 11.9% |
Substrate consumption, biomass and metabolites production rates, for each strain and the different experimental conditions (% O2 in the inlet air: 21, 11, 8). Bottom rows: values of the carbon balance mismatch in percentage including or excluding arabinitol. OUR, oxygen uptake rate; CER, CO2 exchange rate.