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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Nat Genet. 2009 Dec 20;42(1):89–93. doi: 10.1038/ng.494

Figure 1.

Figure 1

Heart defects in epicardial-specific Wt1 mutant embryos. (b) Gata5-Cre+/Wt1 loxP/gfp (Cre+) embryos display edema and accumulation of blood in the systemic veins; a littermate control (Cre−) is shown in panel (a). Scale bars represent 100 μm. (c, d) Haematoxylin and eosin-stained sections of Cre− and Cre + E16.5 embryos. The right ventricle (RV) of some the mutant embryos (d) shows a thinner wall (arrows) as compared to the control (c), while the left ventricle (LV) is apparently normal. Mutant embryos show pericardial haemorrhage (* in d). Scale bars represent 50 μm. (e, f) OPT image of control and mutant hearts at E16.5. Scale bars represent 50 μm. (g, h ) Immunofluorescence staining for the indicated blood vessel markers. Only control embryos show arteries with a well differentiated smooth muscle layer (g). Analysis of EMT markers with antibodies against: Snail (i, j), E-cadherin (k, l), Vimentin and Cytokeratin (m, n). Abnormal E-cadherin (l) and decreased Snail (j) and Vimentin (n) expression is observed in epicardial cells from Cre+ embryos. Scale bars represent 50 μm.

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