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Wt1 is required for EMT in ES cells. (a) Wt1-GFP expression during EB differentiation of Wt1-GFP knockin ES cells. Scale bar represents 50 μm (b) Western Blot (WB) analysis of the endogenous Wt1 protein in EB. (c) WB of epithelial and mesenchymal protein markers in wild type EB (control) and Wt1 KO EB (KO). (d) Phase-contrast microscopy of control and Wt1 KO EB migrating cells. Scale bars represent 50 μm.
