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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Nat Genet. 2009 Dec 20;42(1):89–93. doi: 10.1038/ng.494

Figure 5.

Figure 5

Wt1 is required for the formation of some mesodermal lineage in EB differentiation. RT-PCR analysis for expression of mesodermal (a), endodermal and ectodermal markers (b) in wild type EB (control) and Wt1 KO EB (KO). (c) RT-PCR analysis for expression of Snai1 and Cdh1 in Wt1 KO Snai1 expressing clones (snailc1 and snailc2) versus Wt1 KO GFP expressing clones (c1 and c2). (d) RT-PCR analysis for expression of mesodermal markers in Snai1 clones (Snai1c1 and Snai1c2) versus GFP clones (c1 and c2). (e) Quantification of the number of beating cardiomyocytes in control, and Wt1 KO clones (c1, c2 and Snai1c1, Snai1c2). The graph represents the mean values ±s.e.m from three independent experiments.

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