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. 2009 Dec 8;9:144. doi: 10.1186/1471-2229-9-144

Figure 4.

Figure 4

The N-terminal arginine residues of AtGCSI contain ER localization information. CLSM analysis of Nicotiana tabacum leaf epidermal cells expressing GFP fusions alone (left panels), or co-expressing GFP fusions and the ER marker mRFP-HDEL (middle panels), or co-expressing GFP fusions together with the Golgi marker ST-mRFP (right panels). GCS90 (A) co-localizes with mRFP-HDEL (B, ER in yellow) but not with ST-mRFP (C, ER in green, Golgi in red). When the four arginine residues in position 6, 7, 10 and 12 are replaced by alanine or leucine residues, R/A GCS90 (D-F) or R/L GCS90 (G-I) accumulates exclusively in the Golgi showing that arginine residues are involved in AtGCSI ER localization. Bars = 8 μm.