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The N-terminal arginine motifs are not the unique determinants responsible for ER retention of AtGCSI. When expressed in Nicotiana tabacum leaf epidermal cells Δ13GCS150-GFP is located in the ER (B) A as it was observed for the GCS150 (A) and confirmed after co-expression with the ER marker mRFP-HDEL (C). In contrast, Δ13GCS90-GFP is targeted to the Golgi apparatus (E) where it colocalizes with the Golgi marker ST-mRFP (D) whereas GCS90 is accumulated in the ER (D). Bars: 8 μm.
