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A luminal domain of AtGCSI is sufficient for targeting a Golgi marker into the ER. (A-C) XYLT35 and ST-mRFP are targeted to the Golgi when expressed in Nicotiana tabacum leaf epidermal cells. (D-F, G-I) XYLT35-GCSlum81 or XYLT35-GCSlum60 where co-expressed with ST-mRFP. Both fusion proteins containing a 81 or a 60 aa long luminal domain of AtGCSI fused to the Golgi marker XYLT35 (XYLT35-GCSlum81 and XYLT35-GCSlum60 respectively) are targeted to the ER. Bars = 8 μm.
