Table 2.

Sub-cellular localization of GCS90 after arginine (R) substitutions in the cytosolic tail.

Mutants	Cytosolic domain	Sub-cellular localization
	6 7 10 12
GCS90	M T G A S R R S A R G R I K S S S L-32aa	ER
Δ13GCS90	M K S S S L-32aa	Golgi
Hs10Δ13GCS90	M A R G E R R R R A K S S S L-32aa	ER
GCS13-XYLT35	M T A G A S R R S A R G R I-10aa	ER + GA
CNX11-XYLT35	M N D R R P Q R K R P A-10aa	ER + GA
R/L6-7GCS90	M T G A S L L S A R G R I K S S S L-32aa	ER + punctate structures
R/L10-12GCS90	M T G A S R R S A L G L I K S S S L-32aa	ER + punctate structures
R/L6-12GCS90	M T G A S L R S A R G L I K S S S L-32aa	ER + punctate structures
R/L6-10GCS90	M T G A S L R S A L G R I K S S S L-32aa	ER +GA
R/L7-12GCS90	M T G A S R L S A R G L I K S S S L-32aa	Golgi +ER
R/L7-10GCS90	M T G A S R L S A L G R I K S S S L-32aa	Golgi
R/AGCS90	M T G A S A A S A A G A I K S S S L-32aa	Golgi
R/LGCS90	M T G A S L L S A L G L I K S S S L-32aa	Golgi