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. 2009 Dec 1;9:247. doi: 10.1186/1471-2180-9-247

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Copyright ©2009 Jones et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Electromobility shift assays. A) EMSA gel shift assay with DNA region -1000 - -832 bp upstream of jamA. DNA [270 fmol (= 30 ng)] was assayed with (from left to right) no protein, 7.3 pmol of 7968, 8.4 pmol of GST+5335, or 31 pmol of HctE_IVA. Arrow indicates DNA + protein shift for 7968. B) Serial titration experiment with 45 fmol (= 5 ng) of the same DNA region with (from left to right) no protein, 6.8 pmol, 13.7 pmol, 27.3 pmol, or 54.8 pmol His+7968. Arrow indicates DNA + protein shift.