Table 2. Expression ratios of gene clusters encoding for hydrogenases and assembly genes in M. smegmatis at different growth rates and oxygen concentrations.
| Gene ID | Gene name | Slow/Fast1 | Hypoxia2 2.5% O2 | Hypoxia2 0.6% O2 | Description |
| MSMEG_2262 | hybA | 5.4 | 4.1 | 5.7 | hydrogenase-2, small subunit |
| MSMEG_2263 | hybC | 5.4 | 3.7 | 6.2 | hydrogenase-2, large subunit |
| MSMEG_2264 | 2.3 | 3.5 | 4.4 | peptidase M52, hydrogen uptake protein | |
| MSMEG_2271 | hypB | 1.5 | 2.2 | 2.3 | hydrogenase accessory protein |
| MSMEG_2272 | hypA | 1.9 | 2.8 | 1.8 | hydrogenase nickel insertion protein HypA |
| MSMEG_2273 | hypF | 2.0 | 2.0 | 3.0 | [NiFe] hydrogenase maturation protein HypF |
| MSMEG_2274 | hypC | 1.8 | 2.1 | 2.5 | hydrogenase assembly chaperone HypC/HupF |
| MSMEG_2275 | hypD | 1.8 | 3.0 | 5.2 | hydrogenase expression/formation protein HypD |
| MSMEG_2276 | hypE | 2.0 | 2.0 | 2.0 | hydrogenase expression/formation protein HypE |
| MSMEG_2702 | hypD | 3.6 | 1.4 | 2.4 | hydrogenase expression/formation protein HypD |
| MSMEG_2703 | hypC | 7.0 | 1.5 | 3.5 | hydrogenase assembly chaperone HypC/HupF |
| MSMEG_2705 | hypE | 4.9 | 1.2 | 3.7 | hydrogenase expression/formation protein HypE |
| MSMEG_2711 | hypF | 7.0 | 1.6 | 11.2 | [NiFe] hydrogenase maturation protein HypF |
| MSMEG_2712 | hypC | 15.1 | 1.5 | 12.1 | hydrogenase assembly chaperone HypC/HupF |
| MSMEG_2713 | 14.5 | 1.4 | 8.9 | peptidase M52, hydrogen uptake protein | |
| MSMEG_2718 | 15.4 | 0.9 | 3.5 | iron-sulfur cluster-binding protein, Rieske family | |
| MSMEG_2719 | 18.2 | 1.1 | 6.6 | hydrogen:quinone oxidoreductase | |
| MSMEG_2720 | 27.8 | 1.2 | 3.1 | NADH ubiquinone oxidoreductase, 20 kda subunit | |
| MSMEG_2721 | hypB | 7.9 | 1.0 | 1.0 | hydrogenase accessory protein HypB |
| MSMEG_2722 | hypA | 1.5 | 1.2 | 0.8 | hydrogenase nickel insertion protein HypA |
| MSMEG_3927 | 1.4 | 5.2 | 18.6 | peptidase M52, hydrogen uptake protein | |
| MSMEG_3928 | 2.6 | 2.2 | 7.6 | [NiFe] hydrogenase, alpha subunit, putative | |
| MSMEG_3929 | 1.0 | 8.2 | 26.9 | [NiFe] hydrogenase, delta subunit, putative | |
| MSMEG_3930 | 1.2 | 10.0 | 28.0 | [NiFe] hydrogenase, gamma subunit, putative | |
| MSMEG_3931 | 1.0 | 16.7 | 67.3 | [NiFe] hydrogenase, beta subunit, putative |
Expression ratios with a p-value<0.05 are indicated with bold numbers.
1
Gene expression ratios of slow growing (0.01 h−1) versus fast growing (0.15 h−1) chemostat cultures at 50% oxygen saturation.
2
Gene expression ratios of slow growing (0.01 h−1, 50% oxygen saturation) versus slow growing cultures at either 2.5% or 0.6% oxygen saturation.