Skip to main content
. 2010 Jan 8;5(1):e8640. doi: 10.1371/journal.pone.0008640

Figure 3. Effect of reduction and cysteine mutations on the size of the ETR1 protein complex.

Figure 3

(A) Mutation of Cys4 and Cys6 of ETR1 prevents formation of the disulfide-linked dimer. LPC-solubilized microsomes from plants expressing ETR(wt) or ETR1(C4S,C6S) were incubated in SDS-PAGE loading buffer in the presence or absence of 300 mM DTT, then separated by SDS-PAGE and analyzed by immunoblot. (B) Gel-filtration analysis of wild-type (wt) and cysteine mutants of ETR1. LPC-solubilized microsomes from the indicated plant lines were fractionated on Superose 6HR and the elution profile for ETR1 determined by immunoblot analysis. 5 mM DTT was included in the solubilization and FPLC buffers as indicated.