Figure 7. Effect of ethylene pathway mutations on the size of the ETR1 protein complex.

LPC-solubilized microsomes were fractionated on Superose 6HR and the elution profile for ETR1 determined by immunoblot analysis. Analysis was performed in a line where additional copies of ETR1 was trangenically expressed (tETR1), in a line containing an ethylene-insensitive mutation in ETR1 (etr1-1), and in a line containing a kinase-deficient version of ETR1 (ETR1-G2). Analysis was also performed in lines containing mutations in other members of the ethylene receptor family (the single mutant ers1-2 and the triple mutant etr2/ers2/ein4), and in the downstream pathway components CTR1 and EIN2.