Table 1. Characteristics of the mapping population, linkage map, and selected sequence and gene parameters.
| Chromosome | Total | |||||
| 1 | 2 | 3 | 4 | 5 | ||
| Number of analyzed male F2 hybrid embryos | 112 | 112 | 112 | 112 | 112 | 112 |
| Observed number of recombination events | 106 | 100 | 110 | 99 | 84 | 499 |
| Average number of recombination events per chromosome | 0.95 | 0.89 | 0.98 | 0.88 | 0.75 | 0.89 |
| Observed instances of two/three recombination events per chromosome | 27/0 | 18/0 | 28/1 | 21/0 | 19/0 | 113/1 |
| Average distance between two recombination events (cM) | 49.4 | 49.4 | 50.3* | 39.7 | 39.7 | 46.2 |
| Min/Max distance between two recombination events (cM) | 14.1/86.3 | 23.5/80.9 | 15.5/91.8* | 6.2/65.6 | 17.8/55.5 | 6.2/91.8 |
| Number of mapped markers | 372 | 207 | 188 | 224 | 264 | 1255 |
| Average genetic distance between markers (cM) | 0.3 | 0.4 | 0.5 | 0.4 | 0.3 | 0.4 |
| Number of marker clusters | 61 | 58 | 53 | 41 | 51 | 264 |
| Average distance between marker clusters (cM) | 1.6 | 1.6 | 1.9 | 2.2 | 1.5 | 1.7 |
| Min/Max distance between marker clusters (cM) | 0.9/4.8 | 0.9/6.7 | 0.9/5.7 | 0.9/8.7 | 0.9/4.7 | 0.9/8.8 |
| Average N. vitripennis genotype frequency (%) | 49.2 | 52.5 | 47.5 | 55.0 | 44.4 | 49.5 |
| Min/Max N. vitripennis genotype frequency (%) | 42.0/53.3 | 45.1/56.3 | 44.3/60.7 | 47.3/61.2 | 39.3/55.4 | 39.3/61.2 |
| Genetic length (cM) | 94.5 | 89.8 | 98.9 | 87.6 | 76.1 | 446.9 |
| Mapped scaffolds | 91 | 48 | 46 | 61 | 31 | 265 |
| Mapped scaffolds (orientated) ** | 11 | 10 | 9 | 5 | 7 | 39 |
| Physical length (Mb) | 48.3 | 39.5 | 34.0 | 35.8 | 30.0 | 187.6 |
| GC content (%) | 42.2 | 43.6 | 43.2 | 42.8 | 43.4 | 43.0 |
| Simple repeat content (nt/Kb) | 17.0 | 20.3 | 19.2 | 18.7 | 18.4 | 18.6 |
| Average gene density (genes per Mb) | 59.2 | 58.7 | 49.7 | 59.3 | 66.9 | 58.6 |
| Average gene distance (Kb) | 10.8 | 10.6 | 14.9 | 9.2 | 9.9 | 11.0 |
| Average protein size (aa) | 574.6 | 578.8 | 595.2 | 587.2 | 588.6 | 583.8 |
| Average number of exons per gene | 6.4 | 6.6 | 6.8 | 6.7 | 6.8 | 6.7 |
| Average exon size (nt) | 270.9 | 261.3 | 264.1 | 262.2 | 259.0 | 264.1 |
| Average intron size (nt) | 1324.3 | 1173.0 | 1311.3 | 1265.8 | 1079.5 | 1240.7 |
| Average d A ( N. vitripennis / N. giraulti ) *** | 0.019 | 0.015 | 0.017 | 0.018 | 0.016 | 0.017 |
| Average d A ( N. vitripennis / N. longicornis ) *** | 0.018 | 0.014 | 0.016 | 0.017 | 0.016 | 0.017 |
| Average ω ( N. vitripennis / N. giraulti ) **** | 0.282 | 0.236 | 0.267 | 0.262 | 0.258 | 0.262 |
| Average ω ( N. vitripennis / N. longicornis ) **** | 0.273 | 0.251 | 0.252 | 0.259 | 0.264 | 0.261 |
Characteristics of the studied mapping population of recombinant F2 hybrids of Nasonia vitripennis and Nasonia giraulti, the high-density Nasonia linkage map derived from it, and selected sequence and gene parameters of the mapped sequence scaffolds of the Nasonia genome assembly 1.0.
*
The single instance of three recombination events per chromosome is excluded from the calculation.
**
For misaligned scaffolds, we included them if at least one fragment was orientated.
***
d A = amino acid divergence.
****
ω = ratio of non-synonymous (amino acid replacing) to synonymous (silent) substitution rates.