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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Hepatology. 2010 Jan;51(1):329–335. doi: 10.1002/hep.23335

Figure 4. iPSC-derived hepatic endoderm displays cytochrome P450 metabolism.

Figure 4

iPSC-derived hepatic endoderm were incubated with hepatocyte culture medium supplemented with 50 μM of CYP3A4 or CYP1A2 pGlo TM substrates (Promega) as per manufacturers instructions. 4 hours post-treatment 50 μl of culture medium was removed and read on a luminometer (POLARstar optima). CYP1A2 and CYP3A4 activity is expressed as relative light units (R.L.U.)/ml of tissue culture medium. (n=6). CYP1A2 activity was similar on cells maintained on both ECMs. Luciferase activity is expressed as relative light units (R.L.U.)/ml of tissue culture media. JDM-iPS1 is presented by the first bar in all graphs, PGP9f-iPS1 is represented by the middle bar in each graph and NMF-iPS6 is represented by the last bar in each graph.