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. 2010 Jan 15;6(1):e1000730. doi: 10.1371/journal.ppat.1000730

Figure 3. Identification of pSn-binding (glyco)protein(complexe)s of MARC-145-grown PRRSV and characterization of the pSn-ligand interaction.

Figure 3

(A) Protein A beads were coated with the pSn4D-Fc or pSn4DRE-Fc proteins and incubated with a lysate of MARC-145-grown PRRSV at 37°C to allow binding. The bound and unbound fractions were collected and subjected to non-reducing SDS-PAGE and Western Blot analysis using virus-specific mAbs. (B) MARC-145-grown PRRSV was either treated with sialidase or control-treated and subjected to reducing SDS-PAGE and Western Blot analysis using virus-specific mAbs. (C) MARC-145-grown PRRSV was either treated with sialidase or control-treated, lysed and incubated with pSn4D-Fc-coated protein A beads at 37°C to allow binding. The bound and unbound fractions were collected and subjected to non-reducing SDS-PAGE and Western Blot analysis using a PRRSV GP5-specific mAb.