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. 2010 Jan 15;6(1):e1000721. doi: 10.1371/journal.ppat.1000721

Figure 2. IFNα-induced tyrosine phosphorylation of Janus kinases is inhibited in MARV- but not in EBOV-infected cells.

Figure 2

This inhibition occurs early in infection and is insensitive to PTP inhibitors. (A) Huh-7 cells were infected with MARV or ZEBOV at an MOI of 5 or left uninfected. At 24 h p.i., cells were treated with 1000 IU/ml of IFNα for 20 min where indicated. Cell lysates were analyzed by western blotting using antibodies directed against total protein and phosphorylated forms of Jak1 and Tyk2. (B) Huh-7 cells were infected with MARV or UV-inactivated MARV at an MOI of 5 or left uninfected. 20 min before lysis (24 h p.i.), cells were treated with 2000 IU/ml of IFNα and harvested at the indicated time points. Cell lysates were analyzed by western blotting using antibodies directed against total protein and phosphorylated forms of Tyk2. (C) Huh-7 cells were infected with MARV at an MOI of 5. The cells were treated with DMSO (D) or the phosphatase inhibitors sodium orthovanadate (SO) or PTP1B inhibitor (PTP) prior to IFN treatment (24 h p.i., 2000 IU/ml IFNα for 20 min). Cell lysates were analyzed by western blotting using antibodies directed against total protein and phosphorylated forms of Tyk2. Note that the cuts in the films excised samples irrelevant to this study.