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. 2010 Jan 15;6(1):e1000721. doi: 10.1371/journal.ppat.1000721

Figure 3. MARV VP40 acts as an IFN antagonist.

Figure 3

(A) Vero cells were transfected with 1 µg empty vector (pCAGGS) or the indicated expression plasmids. 24 h post transfection (p.t.) cells were treated with 1000 IU/ml of IFNβ for 24 h and infected with NDV GFP. At 16 h p.i., green fluorescence (indicating viral replication) was visualized and photographed with a fluorescence microscope. (B) Huh-7 cells were transfected with 2 µg of the indicated plasmids expressing the indicated viral proteins from rabies virus, ZEBOV and MARV. At 24 h p.t., the cells were stimulated with 2000 IU/ml of IFNα-2b for 45 min, fixed with 4% paraformaldehyde, and stained with anti-STAT2 antibody and antibodies to detect viral proteins.