Table 1.
Effect of incubation of 2-cell embryos in various conditions for different lengths of times on embryo developmental rates
| Incubation time (h) | Groups | No. of 2-cell embryos | Development (%) | ||
|---|---|---|---|---|---|
| M | E-Bl | H-Bl and Hd-Bl | |||
| 1 | hHTF | 32 | 28 (87) | 24 (75) | 21 (65) |
| pH 6.2 | 32 | 25 (78) | 20 (62) | 17 (53) | |
| pH 8 | 34 | 28 (82) | 21 (62) | 20 (59) | |
| 2 | hHTF | 30 | 28 (92) | 24 (80) | 20 (66) |
| pH 6.2 | 35 | 22 (63) | 17 (49) | 15 (42) | |
| pH 8 | 33 | 25 (76) | 18 (54) | 17 (51) | |
| 3 | hHTF | 30 | 25 (83) | 22 (73) | 19 (63) |
| pH 6.2 | 32 | 12 (37) | 10 (31) | 7 (22)a | |
| pH 8 | 32 | 15 (47) | 12 (37) | 9 (28) | |
| 4 | hHTF | 32 | 26 (81) | 23 (72) | 20 (62) |
| pH 6.2 | 30 | 10 (33)a | 8 (27)a | 5 (17)a | |
| pH 8 | 32 | 12 (37) | 11 (34) | 7 (22)a | |
| 5 | hHTF | 30 | 24 (80) | 19 (63) | 17 (57) |
| pH 6.2 | 33 | 11 (33)a | 4 (12)b | 1 (3.0)c | |
| pH 8 | 34 | 13 (39) | 11 (32) | 5 (17)a | |
Values are pooled from 3 independent experiments. M, morulae at day 3, E-Bl, expanded blastocyst at day 4, H-Bl, hatching blastocyst; Hd-Bl, hatched blastocyst at day 5. a, b, c: P < 0.05, P < 0.01, P < 0.001 (χ2 test) respectively, compared to hHTF (control) at the same developmental stage